gene synthesis Search Results


human u6 promoter  (Danaher Inc)
94
Danaher Inc human u6 promoter
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Human U6 Promoter, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human u6 promoter/product/Danaher Inc
Average 94 stars, based on 1 article reviews
human u6 promoter - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
apparatus t25 hthp synthesis equipment  (Rockland Immunochemicals)
90
Rockland Immunochemicals apparatus t25 hthp synthesis equipment
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Apparatus T25 Hthp Synthesis Equipment, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apparatus t25 hthp synthesis equipment/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
apparatus t25 hthp synthesis equipment - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
plasmid synthesis and subcloning  (GenScript corporation)
90
GenScript corporation plasmid synthesis and subcloning
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Plasmid Synthesis And Subcloning, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid synthesis and subcloning/product/GenScript corporation
Average 90 stars, based on 1 article reviews
plasmid synthesis and subcloning - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gene synthesis  (GenScript corporation)
90
GenScript corporation gene synthesis
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Gene Synthesis, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene synthesis/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gene synthesis - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gene of interest  (BioCat GmbH)
90
BioCat GmbH gene of interest
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Gene Of Interest, supplied by BioCat GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene of interest/product/BioCat GmbH
Average 90 stars, based on 1 article reviews
gene of interest - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
commercial gene synthesis  (GenScript corporation)
90
GenScript corporation commercial gene synthesis
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Commercial Gene Synthesis, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/commercial gene synthesis/product/GenScript corporation
Average 90 stars, based on 1 article reviews
commercial gene synthesis - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gsap gene synthesis service  (Merck KGaA)
90
Merck KGaA gsap gene synthesis service
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Gsap Gene Synthesis Service, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsap gene synthesis service/product/Merck KGaA
Average 90 stars, based on 1 article reviews
gsap gene synthesis service - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gene synthesis  (Gene Design Inc)
90
Gene Design Inc gene synthesis
Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or <t>non-targeting</t> <t>gRNAs</t> is under control of a constitutive human <t>U6</t> (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).
Gene Synthesis, supplied by Gene Design Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene synthesis/product/Gene Design Inc
Average 90 stars, based on 1 article reviews
gene synthesis - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
species-optimized synthetic genes  (General Biosystems Inc)
90
General Biosystems Inc species-optimized synthetic genes

Species Optimized Synthetic Genes, supplied by General Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/species-optimized synthetic genes/product/General Biosystems Inc
Average 90 stars, based on 1 article reviews
species-optimized synthetic genes - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gene synthesis  (Eurofins)
90
Eurofins gene synthesis

Gene Synthesis, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene synthesis/product/Eurofins
Average 90 stars, based on 1 article reviews
gene synthesis - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gene synthesis  (Biotechnology Information)
90
Biotechnology Information gene synthesis

Gene Synthesis, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene synthesis/product/Biotechnology Information
Average 90 stars, based on 1 article reviews
gene synthesis - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
full-gene synthesis  (Blue Heron Biotech)
90
Blue Heron Biotech full-gene synthesis

Full Gene Synthesis, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/full-gene synthesis/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
full-gene synthesis - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or non-targeting gRNAs is under control of a constitutive human U6 (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).

Journal: Molecular Therapy. Nucleic Acids

Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene

doi: 10.1016/j.omtn.2023.02.033

Figure Lengend Snippet: Structure of the recombinant adenovirus vector constructs and gRNA sequences incorporated into them (A) Schematic of HAdV-5-based vectors with inserted individual gRNA sequences. All adenoviral vectors are based on the HAdV-5-derived vector pAd/PL-DEST (Thermo Fisher Scientific) and lack the E1 and E3 regions. Cas9 and gRNA expression cassettes were inserted into the deleted E1 region. The expression of spCas9-HF1 is driven by a tetracycline repressor-controlled CMV promoter comprising two binding sites for the repressor (2×TetO2). The expression of the individual targeting or non-targeting gRNAs is under control of a constitutive human U6 (hU6) promoter. The structure and sequence of the gRNAs are exemplarily shown for E1A gRNA 9 bound to its target site. The control vector containing only the Cas9 expression cassette is also depicted. (B) Target sequences for the individual gRNAs and their positions within the HAdV-5 genome (AY339865.1).

Article Snippet: Linear DNA fragments (gBLOCKS) containing individual targeting or non-targeting gRNAs under control of a human U6 promoter were generated by gene synthesis (Integrated DNA Technologies, Coralville, IA, USA), and the individual expression cassettes were inserted into the Hind II and NotI sites of the SpCas9-HF1 expression cassette-containing intermediate vector, giving rise to vectors pENTR-CMV-TetO2-spCas9-HF1-hU6-gRNA 1–10, containing a single targeting gRNA each, and to the control vectors containing non-targeting gRNAs (pENTR-CMV-TetO2-spCas9-HF1-hU6-NT gRNA) or containing only the spCas9-HF1 expression cassette (pENTR-CMV-TetO2-spCas9-HF1).

Techniques: Recombinant, Plasmid Preparation, Construct, Derivative Assay, Expressing, Binding Assay, Control, Sequencing

Journal: eLife

Article Title: Enzyme activity and selectivity filter stability of ancient TRPM2 channels were simultaneously lost in early vertebrates

doi: 10.7554/eLife.44556

Figure Lengend Snippet:

Article Snippet: Gene ( Salpingoeca rosetta TRPM2) , srTRPM2, srNUDT9-H , General Biosystems, Inc. , NCBI: XP_004993318 , species-optimized synthetic genes.

Techniques: Plasmid Preparation, Mutagenesis, Avidin-Biotin Assay, Software